Isolation and identification of fungal communities from vegetable wastes composts,

Compost preparation was carried out at Department of Botany, Bangalore University Bangalore during 2013 and 2014 by using Cabbage, tomato fresh cow dung and mixed vegetable waste. The experiments were laid down in a completely replicated randomized block design with three replication for each treatment. The qualitative and quantitative composition of individual vegetable composts (Tomato waste, Cabbage waste and mixed vegetable waste) were used for the isolation. The fungi were isolated on Potato Dextrose Agar (PDA) and at different temperature 50-55°C. Substantially qualitative differences in the species composition of different vegetables composts were observed. A total of 11 entities were isolated 3 from tomato waste compost, 2 from cabbage waste compost and 4 from mixed vegetable waste at 50°C and 3 at 55°C respectively. This report demonstrates that the qualitative and quantitative characterization of individual composts and fungal communities essential for best industrial application

MOLECULAR DOCKING STUDIES ON THIADIAZOLE DERIVATIVES AS PROTEIN KINASE INHIBITORS

Objective: In the present study, a novel series of 1, 3, 4-thiadiazole derivatives were docked against the mycobacterium tuberculosis protein kinase G. 1, 3, 4–thiadiazole derivatives with a modified primary amine group at 5th position were used for docking studies. Methods: The three-dimensional structure of the protein was obtained from PDB, and its active sites were predicted. The structures of all the compounds were drawn using chemdraw software version 8.0. The docking studies were done by using schrödinger software against the enzyme protein kinase G. Totally eighteen compounds was synthesized based on glide score Results: In this Docking study the thiadiazole analogues were showing good binding energy. The amino acids residues GLU588, SER412, GLY410 and GLU 628 in the kinase domain active site form hydrogen bonds with the ligand. Conclusion: The compounds D34, D16, D7, D25, D15, and D27 showed better interaction with protein kinase G (pknG) more than the other drug molecule

COMPARATIVE STUDY ON ANTIMICROBIAL ACTIVITY OF SEAWEEDS

Objective: Secondary metabolites from natural resources are a potential source of antimicrobial leads and drugs can exploited to combat antimicrobial resistance in microorganisms. Seaweeds are considered as a valuable source with a broad spectrum of biological activities. Hence, this study was undertaken to screen seaweeds from Mandapam coastal waters, East coast of India, for antimicrobial activity.Methods: Compounds were extracted using methanol from the seaweeds, namely, Halimeda gracilis, Caulerpa serrulata, Sargassum swartzii, Sargassum wightii, Jania rubens, Ulva lactuca, Ulva fasciata, Gracilaria corticata, Stoechospermum marginatum, Caulerpa scalpelliformis, Caulerpa taxifolia, Chaetomorpha crassa, Enteromorpha flexuosa, and Turbinaria ornate. The extracts were screened for their antimicrobial activity against selected bacterial and fungal pathogens.Results: In the present study, S. swartzii, J. rubens, and S. marginatum showed broad spectrum of antibacterial activity against all the test bacterial pathogens. Among these, the maximum activity was exhibited by S. swartzii against Enterococcus faecalis (27.00 ± 0.88) and Streptococcus pyogenes (23.00 ± 0.84), followed by J. rubens against E. faecalis (26.00 ± 0.56) and S. pyogenes (22.00 ± 0.75), and S. marginatum exhibited significant inhibition against Staphylococcus aureus (15.00 ± 0.22) and S. pyogenes (18.00 ± 1.16).Conclusion: These seaweeds with significant antibacterial activity will subjected to phytochemical screening to find out the potential active principle responsible for antimicrobial activity. It is followed by purification and characterization of the compounds for possible application in drug formulation, can take this to large-scale application in pharmaceutical industries

Study of the obstetrics and perinatal outcome of in vitro fertilization pregnancies at tertiary care centre

Background: Assisted reproductive techniques (ART) has been a boon to the unfortunate. However, the outcomes aren’t the same to all. The study has observed the outcomes in IVF conceived patients. The pregnant women were followed-up throughout their antenatal and post-natal (7 days) period and an appropriate meta-analysis was made.Methods: This is an Observational Prospective study. It was conducted at an IVF center of tertiary hospital. With valid written informed consents 96 patients were recruited and observed for18 months. Data collected by Universal sampling method, analyzed and interpreted.Results: A total 65.6% belonged above 30 years, 67.7% were primigravida and 7.3% had history of previous ectopic pregnancy. In 23.75% multifetal pregnancy was significant and 20.63% had OS tightening done. Most common reason for opting IVF was multiple failed IUI (71.6%). More than 40% were delivered by caesarean section. 91.1% of babies cried immediately after birth and 50.4% babies required NICU admission.13.4% babies had neonatal death and 3.1% were IUFD. 58.30% pregnancies were singleton. Mean birth weight was 1.87 Kg and 72.6% children were born low birth weight. Statistical significance (p<0.001) was found in between no. of foetuses delivered and preterm delivery. 56.3% birth were preterm birth. PIH and GDM complication is most common in multiple gestations observed in 10.7% of pregnancies.Conclusions: Assisted Reproductive technologies has helped the infertile couples. Outcomes could be unfruitful in few, while others may have a complicated antenatal period. Preterm deliveries are more common in multiple gestation thus complementing the lower birth weights. Comorbidities significantly affected obstetric outcome

Synthesis and Characterization of Silica from Ragi Husk Ash (Finger Millet) by a Sol-gel Method

Silica aerogel, a mesoporous material, was prepared from ragi husk ash by sol–gel method and dried under atmospheric pressure. In this method, ragi  husk ash, which is rich in silica, was extracted with sodium hydroxide solution to produce a sodium silicate solution. This solution was neutralized with acid to form a silica aerogel. The aged gel was washed carefully by de ionized water and ethanol and finally dried under atmospheric air. A temperature of 7000C was found to be optimum ashing temperature with maximum amorphous silica content. From a morphological analysis obtained by SEM and diffraction patterns (XRD), a longitudinal fibrous and amorphous structure was observed for Ragi Husk. FTIR characterization indicated the presence of silanol and siloxane groups. Thermo gravimetric was used to characterize burning behaviour and also to determine the activation energies. This economic technology, as applied to waste material, also provides many benefits to the local agro-industry. Keywords: Ragi husk Ash, Sol- gel method, Amorphous silica, Aeroge

Reproductive potential in a case of Mosaic Turners syndrome with 46XY karyotype

This article describes a case of 18year-old-female who presented with primary amenorrhea, phenotypic features of Turners syndrome, which was confirmed later by Karyotype to have mosaic 45XO(8)/46XY(22). She had delayed puberty and proved (hormonally) to have ovarian failure, with absent Mullerian structures (radiologically and laparoscopy)

Assess the effectiveness of video assisted teaching about hazards of plastics and its safe disposal among the housewives residing at selected urban area at Choolai in Chennai

Community health education services are an economical and powerful means of raising standard of community health, especially for the future generations. Community is considered as a best setting for the positive health and prevention of diseases, awakening health consciousness in which the country grows and develops. A pre experimental study was conducted to assess the effectiveness of video assisted teaching about hazards of plastics and its safe disposal among the housewives residing at selected urban area at Choolai in Chennai. Totally 100 housewives, those who met the inclusion and exclusion criteria were selected by convenient sampling technique. A pretest was conducted by using a structured questionnaire, followed by video assisted teaching program. After 7 days posttest was taken. The result of the posttest score revealed that the video assisted teaching program had its impact on improving the level of knowledge regarding hazards of plastic and its safe disposal among housewives. The overall mean score of pre-test was 10.46 with the S.D. 3.84, whereas in post-test the overall mean score was 17.98 with S.D. 1.74. The t-test value was 24.03 which is statistically significant at p<0.005 level of significance. After video assisted teaching program none of the housewives were having inadequate knowledge, 19.0% of them were having moderate knowledge and 81% of them were having adequate knowledge and the score is statistically highly significant (p=0. 001). The study finding implied that the education had a vital role in improving the knowledge of housewives regarding hazards of plastic and its safe disposal. Housewives play a vital role in family and in community and what they learn is likely to be applied during the rest of their lives thereby they can bring a big change for a better future

Growth of Nursery Grown Micro Propagated Bamboo (Bambusa Tulda .L) Inoculated with Arbuscular Mycorrhizal Fungus and Plant Growth Promoting Rhizobacteria (PGPR)

A nursery experiment was conducted to investigate the effect of bio inoculants AMF (Glomus mosseae) Frateuria aurantia (potassium mobilizer), Azospirillium (Free nitrogen fixer) on growth promotion of micro propagated thorn less bamboo (Bambusa tulda .L).Bamboo seedlings were inoculated singly or in triple inoculation with Glomus mosseae, Frateuria aurantia and Azospirillium brasilense. The mycorrhizal spore load and per cent root colonization were maximum with triple inoculated plants and least in control and uninoculated plants. - See more at: http://www.worldwidejournals.com/international-journal-of-scientific-research-(IJSR)/articles.php?val=MzMzNw==&b1=89&k=23#sthash.tKy9r1Nv.dpu